During the operation of subway vehicles, the vibration of air conditioning units is mainly transmitted to the vehicle body through the suspension support, which seriously affects the stability and comfort of the vehicle during operation. Therefore, the design and optimization of the suspension support of air conditioning units has become a hot topic in the research of the dynamic characteristics of subway vehicles. In this paper, the rigid and flexible coupling dynamic model of metro is firstly calculated to simulate the stress of the suspension point of air conditioning of the vehicle body when the vehicle is running. The initial structure design of the suspension support is carried out, and the stress of the air conditioning suspension point is taken as the load input to analyze the stiffness and strength of the initial structure of the suspension support. Then, the fatigue life is taken as the topology constraint, and the variable density method (SIMP) is used to optimize the topology of the suspension bracket. Finally, the optimized suspension support is validated. The results show that after topological optimization, the maximum displacement and maximum stress of the suspension support under vertical, horizontal, and vertical loads are reduced by 80%, 93%, and 99%, respectively, compared with the original structure model, and the maximum stress under vertical loads is reduced by 50%.