The proposal of this study is a protocol for the preparation of permanent sheets of protists and rotifers by adapting a Kisser glyceryn-jelly technique in three stages. A total of 450 sheets of protists and rotifers were made. After a period of 2 years, the slides remained stable, with no signs of fungal contamination and the organisms maintained their integral morphological conformation. The proposed technique is promising for the conservation of the morphological characteristics of the specimens, presenting low risk of manipulation, low cost, few reagents and easy handling, allowing the creation of a didactic collection for practical classes.