Published in
American Society for Microbiology, Journal of Clinical Microbiology, 3(59), 2021
DOI: 10.1128/jcm.02078-20
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Development of a Duplex Real-Time PCR Assay for the Differentiation of Blastomyces dermatitidis and Blastomyces gilchristii and a Retrospective Analysis of Culture and Primary Specimens from Blastomycosis Cases from New York (2005 to 2019)
Journal article published in 2021 by
Mitchell Kaplan,
YanChun Zhu,
Julianne V. Kus 
,
Lisa McTaggart,
Vishnu Chaturvedi ,
Sudha Chaturvedi
,
Lisa McTaggart,
Vishnu Chaturvedi ,
Sudha Chaturvedi
This paper is made freely available by the publisher.
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Abstract
Blastomycosis due to Blastomyces dermatitidis and Blastomyces gilchristii is a significant cause of respiratory mycoses in North America with occasional reported outbreaks. We developed a highly sensitive, specific, and reproducible TaqMan duplex real-time PCR assay for the differentiation of B. dermatitidis and B. gilchristii .