ABSTRACT Sugarcane cultivation areas are usually characterized by low soil fertility and high nutrient demand, especially for nitrogen. Technologies that can raise crop yield and lower the use of pesticides and fertilizers are among the main needs of this crop. The objective of this study was to evaluate the agronomic performance of sugarcane after in-furrow application of the diazotrophic bacterium Nitrospirillum amazonense (BR11145). The treatments consisted of applying five doses of N. amazonense-based product to the planting furrow, 0.25, 0.5, 1.0, 1.5, and 2.0 L commercial product (c.p.) ha-1; a treatment consisting of the combination of Bacillus subtilis and Bacillus licheniformis; one control without fertilization; and one control with fertilization. Based on the results obtained from the pooled analysis of two field experiments, the N. amazonense-based product improved the growth parameters of sugarcane plants, and at doses of 1.0 and 1.5 L c.p. ha-1, these benefits resulted in a significant increase in yield and in the amount of sugar per hectare. Inoculation with diazotrophic bacteria can benefit sugarcane, especially because it promotes the growth and yield of the crop similarly to fertilizers.