Objective: This study compare the prevalence of urinary tract infection among kidney transplanted and other UTI suspected patients visiting National Public Health Laboratory, Teku, Nepal Materials and methods: The study was carried out from January 2011 to October 2011. During this period, a total of 1233 urine samples from patients suspected of UTI were collected and processed. The antimicrobial susceptibility was tested by the modified Kirby-Bauer’s disc diffusion method as per the CLSI guidelines. All the data obtained was statistically analyzed by using Statistical Package for Social Sciences (SPSS) version 16. Results: Prevalence of uropathogens was found to be 14.19% (175/1233). 16 species of different bacterial isolates were isolated, identified and among them, 94.29% (165/175) were of Gram negative and 5.71% (10/175) of Gram positive organisms. Out of total 439 Kidney transplanted patients, only 5.01% (22) of urine sample showed significant growth. The most efficient first line antibiotic for isolates was found to be Ceftriaxone 68.57%, and in second line antibiotics Ceftazidime-clavunic acid and Amikacin showed better efficacy. In the total 175 uropathogens, 48% (84/175) isolates were found to be MDR isolates. Conclusion: A 14.19% prevalence of UTIs was found. 94.29% (165/175) of Gram negative uropathogens were found to be predominant. This study showed the significant association between infection among Kidney transplanted and Non-transplanted patients (p