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Nature Research, Nature Structural and Molecular Biology, 6(20), p. 763-765, 2013

DOI: 10.1038/nsmb.2574

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CENP-A confers a reduction in height on octameric nucleosomes

Journal article published in 2013 by Matthew D. D. Miell, Colin J. Fuller, Helena M. Barysz, Annika Guse, Andrew Downes, Tom Owen-Hughes, Juri Rappsilber, Robin C. Allshire ORCID, Aaron F. Straight
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Nucleosomes in which histone H3 is replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes extracted from human and Drosophila cells have been reported to have reduced heights relative to canonical octameric H3 nucleosomes, suggesting a unique tetrameric, hemisomal composition. We demonstrate that even octameric CENP-A nucleosomes assembled in vitro exhibit a reduced height, indicating that they are physically distinct from H3 nucleosomes, and negating the need to invoke the presence of hemisomes.