AbstractVasocative-intestinal-peptide (VIP⁺) and somatostatin (SST⁺) interneurons are involved in modulating barrel cortex activity and perception during active whisking. Here we identify a developmental transition point of structural and functional rearrangements onto these interneurons around the start of active sensation at P14. Using in vivo two-photon Ca²⁺ imaging, we find that before P14, both interneuron types respond stronger to a multi-whisker stimulus, whereas after P14 their responses diverge, with VIP⁺ cells losing their multi-whisker preference and SST⁺ neurons enhancing theirs. Additionally, we find that Ca²⁺ signaling dynamics increase in precision as the cells and network mature. Rabies virus tracings followed by tissue clearing, as well as photostimulation-coupled electrophysiology reveal that SST⁺ cells receive higher cross-barrel inputs compared to VIP⁺ neurons at both time points. In addition, whereas prior to P14 both cell types receive direct input from the sensory thalamus, after P14 VIP⁺ cells show reduced inputs and SST⁺ cells largely shift to motor-related thalamic nuclei.