Abstract Introduction Considering the increased search for esthetic procedures, questions emerge about the maintenance and regression of color, especially regarding the need to restrict dark foods during bleaching procedures or their influence on the stability and effectiveness of the treatment. Objective To assess the influence of staining agents on tooth enamel during immediate bleaching treatment with 35% hydrogen peroxide. Material and method Sixty bovine teeth were divided into six groups (G) (n=10): G1: distilled water (control); G2: coffee; G3: cola soft drink; G4: wine; G5: mate tea; and G6: industrialized açaí. The samples were subjected to immediate bleaching using 35% hydrogen peroxide for 21 days (three applications of 15 minutes per session, every seven days) and pigment immersion between each session for 15 minutes. The color was assessed before starting bleaching and after 21 days, using a spectrophotometer. The data obtained were subjected to one-way ANOVA and heteroscedasticity analysis by the Welch and Brown-Forsythe tests. The Tamhane test was used for group comparison. Result The color change was visible to the naked eye, considering the mean ΔE in all groups tested was higher than 3.7. However, there was no statistical difference between the control group and the groups assessed. Conclusion The contact of staining agents on bovine tooth enamel did not affect the final result of the immediate tooth bleaching.