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American Society for Microbiology, Journal of Clinical Microbiology, 12(58), 2020

DOI: 10.1128/jcm.01553-20

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The Isolation of Orientia tsutsugamushi and Rickettsia typhi from Human Blood through Mammalian Cell Culture: a Descriptive Series of 3,227 Samples and Outcomes in the Lao People’s Democratic Republic

Journal article published in 2020 by Damien K. Ming, Vanheuang Phommadeechack, Phonepasith Panyanivong, Davanh Sengdatka, Weerawat Phuklia, Vilada Chansamouth, Tamalee Roberts ORCID, Stuart D. Blacksell ORCID, Paul N. Newton, Matthew T. Robinson ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

In the Lao People’s Democratic Republic (Laos), rickettsial infections, including scrub and murine typhus, account for a significant burden of fevers. The Mahosot Hospital Microbiology Laboratory in Vientiane, Laos, routinely performs rickettsial isolation from hospitalized patients with suspected rickettsioses using mammalian cell culture systems. We review the clinical and laboratory factors associated with successful Orientia tsutsugamushi and Rickettsia typhi isolations from this laboratory over a period of 6 years between 2008 and 2014.