Objective: to construct and validate a questionnaire for tracking disciplines and content of Forensic Nursing and similar disciplines in undergraduate courses. Methods: methodological study for the construction and validation of content and semantics. The items were constructed and categorized using content analysis, based on the national and international theoretical framework of Forensic Nursing, structured in three sections with dichotomous and subjective questions. Content and semantics were validated using the brainstorming technique and an 80% concordance index among the judges. Results: the sections were: identification of courses, of disciplines of Forensic Nursing and Violence, and contents of Forensic Nursing discipline. Conclusion: the Forensic Nursing Discipline and Content Supervision Questionnaire for Graduation Nursing Courses was considered easy to use and dynamic to assess the situation of Forensic Nursing and Violence-related subjects in Nursing courses at college Institutions.