Isomucor trufemiae was isolated and described for the first time from soil samples collected in the state of São Paulo State, Brazil, in 2012. Eight years later, we isolated this species in the state of Pernambuco as the second record worldwide and the first record to northeastern Brazil. Isomucor trufemiae URM 8342 was isolated from a soil sample during a study on the diversity of Mucorales in a Montane Atlantic Forest area in the municipality of Bonito, Pernambuco, Brazil, and identified through morphological and molecular analyses (ITS and LSU sequences of rDNA). Aspects of the morphology and distribution of this species are commented in this manuscript.