Abstract The biogas production characteristics of anaerobic fermentation of cattle manure and corn stover with different proportions were studied to provide basis for improving efficiency. The dry matter mass ratio of corn stover and cattle manure was set as 1:1,1:2,1:3 and 2:1 respectively. The anaerobic co-digestion was carried out under the fermentation conditions of 8% TS and medium temperature (35±1°C), analysing the change rule of each biomethane production index. The results showed that when the mass ratio of corn stover to cattle manure was 2:1, daily biogas production was up to 80 mL; compared with 1:2 and 1:3, the maximum cumulative biomethane production volume of 4540mL increased by 21.67% and 30.12% respectively. The highest volume fraction of methane is 72%.