ABSTRACT This study presents an analysis of linguistic aspects at the segmental and suprasegmental levels in individuals with Down syndrome with or without a diagnosis of speech apraxia. Ten individuals of both sexes, aged between 13 and 32 years, participated in the study. Data collection was performed, individually and separately, in a video recorded therapeutic session. Speech tasks consisted of word repetition, repetition of sentences and automatic speech. The speech samples were submitted to phonetic transcription with a description and analysis of phonoarticulatory alterations, typology of disfluencies and prosodic alterations. The data were submitted to descriptive and inferential statistical analysis, using the Mann-Whitney test for independent samples and considering p-value≤0.05 as significant. Individuals with speech apraxia (n=6), compared with those without it (n=4), presented a higher occurrence of phonoarticulatory alterations, with a statistically significant difference between the two groups in omission (p=0.018) and articulatory inaccuracy (p=0.030) alterations; a higher occurrence of disfluencies, mainly of the syllable repetition type; and the occurrence of prosodic alterations (83.3%), which was not found in the group without speech apraxia. The importance of the differential diagnosis of speech disorders in Down syndrome is revealed with an evaluation that considers the different linguistic aspects resulting from the differentiation of the characteristics of speech. Clinical intervention should be early and guided by specific parameters.