The tomato is a species of undetermined growth and extremely demanding in nutrients Therefore, the search of new ways to maximize the use and supply of fertilizers sources and their application mechanisms are important to improve the tomato culture management. The aim of this study was to evaluate the application methods of liquid biofertilizer (BF) and the harvest times throughout cultivation. The experiment was carried out in a randomized block design, in a 3x6 factorial with subdivided plot and seven replications. The treatments were foliar and drip BF application and a control, without application of BF, and harvesting time (85; 92; 99; 106; 113; 120 days after transplanting-DAT). Leaf application resulted in a class I production increase in the second week of harvest (92 DAT), while drip application reflected higher class II and III production in the fourth week (106 DAT). In all treatments, at 92 DAT higher production of large fruits (class I) was observed. Production of average fruits (class II) occurred at 92 and 113 DAT and small fruit (class III) production was concentrated at 113 DAT. The adoption of BF, regardless of the application form, provides an increase in total productivity, with an income up to 35% higher. Therefore, biofertilizer is a good source for nutrition implementation aiming at yields and returns in the tomato production chain.