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The disease caused by SARS-CoV-2 has affected many countries and regions. In order to contain the spread of infection, many countries have adopted lockdown measures. As a result, SARS-CoV-2 has negatively influenced economies on a global scale and has caused a significant impact on the environment. In this study, changes in the concentration of the pollutant Nitrogen Dioxide (NO2) within the lockdown period were examined as well as how these changes relate to the Spanish population. NO2 is one of the reactive nitrogen oxides gases resulting from both anthropogenic and natural processes. One major source in urban areas is the combustion of fossil fuels from vehicles and industrial plants, both of which significantly contribute to air pollution. The long-term exposure to NO2 can also cause severe health problems. Remote sensing is a useful tool to analyze spatial variability of air quality. For this purpose, Sentinel-5P images registered from January to April of 2019 and 2020 were used to analyze spatial distribution of NO2 and its evolution under the lockdown measures in Spain. The results indicate a significant correlation between the population’s activity level and the reduction of NO2 values.