ABSTRACT Knowledge on the water relations and biochemical responses of plants has been pointed out as important for selecting genotypes that are tolerant to abiotic stresses, such as drought and salinity. Thus, the objective of this study was to evaluate the leaf water potential, enzymatic activity and yield of sugarcane (RB 92579) irrigated with brackish waters and leaching fractions. The study was conducted from November 2016 to November 2017 in Recife, PE, Brazil. The experimental design was completely randomized, in a 5 x 2 factorial scheme, with four repetitions. Treatments consisted of five irrigation water salinity (0.5; 2.0; 4.0; 6.0 and 8.0 dS m-1) and two leaching fractions (0 and 0.17), corresponding to 100 and 120% of crop evapotranspiration. The values of irrigation water salinity were obtained by adding NaCl and CaCl2 at molar proportion of 1:1 (Ca:Na) to water from the local supply (ECw = 0.5 dS m-1). Readings of leaf water potential (Ψw) were taken at predawn, and +3 leaves were collected for the determination of catalase and ascorbate peroxidase activity at 90, 150, 210 and 270 days after planting. Yield was determined at 365 days after planting. Increase in water salinity reduced leaf water potential and yield, and increased the activity of catalase and ascorbate peroxidase, and the leaching fraction of 0.17 was able to reduce the deleterious effects of salts on plants.