With the depletion of fossil fuel resources accelerating the search for renewable energy sources, studies on agricultural products containing a significant amount of oil in their compositions have intensified. The objective of this work was to investigate the fatty acid profile and the physiological quality of the oil extracted from niger seeds dried at different temperatures. The seeds were dried at 40, 50, 60, and 70°C, until their moisture content reached 8.5 ± 1% (wet basis). The physiological properties of niger seeds were determined by assessing their germination, germination speed index, mean germination time, cold test, accelerated aging with a saturated solution, emergence percentage, and emergence speed index. The quality of the oil was evaluated using gas chromatography, based on the fatty acid profile. We concluded that drying air temperatures of 40 and 50°C did not compromise the physiological quality of niger seeds. However, the 70°C drying temperature seriously impaired seed quality. The drying temperatures that were used in this study did not substantially alter the fatty acid profile of niger oil.