The Hulun Buir sandy land in northern China is located at the northern limit region of the East Asian summer monsoon (EASM) and is therefore sensitive to the extension of the front of the rainfall belt. Here we report an n-alkane and compound-specific carbon isotope record from the Holocene sediments of Lake Yiheshariwusu in the middle of the Hulun Buir sandy land. The sediments contain a suite of n-alkanes with a strong odd over even carbon number predominance, with the maximum contribution from nC₃₁, which is a typical distribution in grassland regions. The low temperatures in this cold region greatly limit the growth of C4 plants and thus the long-chain n-alkanes in lake sediments are mainly derived from leaf wax lipids of C3 plants growing within the sandy land. In this C3-vegetation-dominated region, the δ¹³C_27–33 value (weighted carbon isotope values of nC₂₇– nC₃₃) are regulated mainly by the physiological and biochemical responses of plants to water stress and are therefore interpreted as a proxy of effective precipitation or humidity. The δ¹³C_27–33 time series shows a trend of gradually decreasing values that suggests an increase in effective precipitation since 8.5 ka (1 ka = 1000 cal yr BP). Relative droughts occurred during the intervals of 6.3–5.5, 4.1–3.6 ka, and during the last 200 years. In addition, the δ¹³C_27–33 time series and comparable paleoenvironmental records from neighboring sites suggest opposite trends of summer monsoon rainfall between northeastern and southeastern China. We suggest that a coupled process between low and high latitudes (the western Pacific Subtropical High and the Okhotsk High) may have played a fundamental role in regulating the shift of the frontal rainfall belt and monsoon rainfall distribution in eastern China during the Holocene.