There is limited research regarding proper fertilization rates and timing for landscape-grown herbaceous perennials. Most current nitrogen (N) fertilizer recommendations for landscape-grown perennials are based on rates for woody landscape plants or on rates for greenhouse-grown perennials. In addition, most fertilizer guidelines are defined to achieve peak growth, which may not be the best indicator of desirable plant quality. Basing fertilizer input rates on plant quality levels rather than maximum growth may result in a lower fertilizer application rate and a reduction in excess fertilizer available for leaching. The objective of this research was to evaluate the response of landscape-grown herbaceous perennials to N fertilizer applied at five rates. Five herbaceous perennials [bush daisy (Gamolepis chrysanthemoides), ‘New Gold’ lantana (Lantana ×hybrid), ‘Mystic Spires’ salvia (Salvia longispicata ×farinacea), ‘Evergreen Giant’ liriope (Liriope muscari), and ‘White Christmas’ caladium (Caladium bicolor)] were transplanted into raised landscape beds containing subsoil fill in U.S. Department of Agriculture (USDA) hardiness zone 9a. Controlled-release N fertilizer was applied at an annual N rate of 0, 2, 4, 6, and 12 lb/1000 ft² for 96 weeks. Plant size index (SI), tissue chlorophyll, and plant quality were measured every 6 weeks for 96 weeks. Flower cover was determined every 6 weeks from 42 to 96 weeks. Shoot biomass and tissue total Kjeldahl N (TKN) were measured at 96 weeks after planting (WAP). Regression analyses suggested that some species required in excess of 12 lb/1000 ft² N to reach maximum size, chlorophyll content, and shoot biomass. However, plants exhibited quality ratings of good to excellent at annual N rates of 2 to 4 lb/1000 ft² N per year. We suggest that these low to moderate levels of N fertilization (2 to 4 lb/1000 ft² N per year) will provide sufficient N to produce acceptable size and quality herbaceous perennials in the landscape.