Significance This study demonstrates how different lines of evidence from tree rings in widely spread growth locations can combine to fix an approximately dated tree-ring record from the East Mediterranean Bronze–Iron Age to an exact calendar-dated range. This tree-ring record is of high importance for regional chronology and spans the time period in which the major volcanic eruption of Thera (Santorini) occurred. Exact dating of this eruption is important because it provides a prominent marker horizon through which ancient timelines of the East Mediterranean, Egypt, and the Levant can be synchronized. Chemical analysis of the dated tree-ring sequence identifies a chemical change in their growth environment around 1560 BC, which while requiring further substantiation, may be evidence of the Thera eruption.