Papaya (Carica papaya L.) is one of the leading fruit trees in Brazil, mainly in the Northeast region. However, some regions suffer from water scarcity, making wastewater reuse a viable alternative for crop production. Also, biostimulants may be used to maximize papaya growth and development. Thus, this work aimed to evaluate the use of Acadian® biostimulant in the growth of Formosa papaya seedlings irrigated with different concentrations of fish-farming wastewater. The experiment was block randomized in a 2 x 5 factorial scheme, use and non-use of biostimulant and five concentrations of wastewater (0, 10, 20, 30, and 40%) diluted in potable water, with four replicates. Qualitative data were compared by t-test at 5% probability, and quantitative data were submitted to regression analysis. Results showed that high concentrations of wastewater negatively affect the production of Formosa papaya seedlings. Acadian® negatively influences plant height, number of leaves, and the ratio between shoot and root. However, the seaweed biostimulant positively influenced the chlorophyll content index.