Abstract Origanum vulgare L. is an aromatic enduring herb that belongs to Lamiaceae family. The bioactive constituents of this herb, such as carvacrol and thymol possess several medicinal properties, such as antioxidant, antidiabetic, anti-inflammatory, antimicrobial, antiviral, antiparasitic, anti-neoplastic, and immune modulatory. Moreover, it is considered a standard natural, less toxic, and residue free feed additive, that is successfully used in livestock and fish. Additionally, in human, Origanum vulgare is extensively used with promising health benefits against respiratory, digestive and urinary disorders. This review casts light on description, chemical composition and structure of Origanum vulgare, as well as its therapeutic applications in human and its biological activities in ruminants and fish, data that will be possibly useful for physiologists, nutritionists and veterinarians.