The sedentary root-knot nematodes, Meloidogyne spp., and the migratory root-lesion nematodes, Pratylenchus spp., cause significant yield losses, particularly in aerobic and upland rice production systems. Recently, the Asian rice Oryza sativa accessions LD 24 and Khao Pahk Maw (KPM) were shown to be highly resistant to M. graminicola. In this study, we have analyzed the responses and broadness of resistance of these two rice genotypes to another root-knot nematode M. javanica and a root-lesion nematode P. zeae. The penetration as well as post-penetration development and reproduction of nematodes were compared including known susceptible and resistant genotypes. Our results indicate that the genotype KPM confers strong resistance to both M. javanica and P. zeae, while LD 24 was resistant to M. javanica and susceptible to P. zeae. Detailed observations revealed that similar numbers of M. javanica or P. zeae penetrated the resistant and susceptible hosts during early infection stages. However, the development and reproduction of both nematodes were arrested or reduced in resistant genotypes, implying that resistance occurs at the post-penetration stage.