Using drones to count wildlife saves time and resources and allows access to difficult or dangerous areas. We collected drone imagery of breeding waterbirds at colonies in the Okavango Delta (Botswana) and Lowbidgee floodplain (Australia). We developed a semi-automated counting method, using machine learning, and compared effectiveness of freeware and payware in identifying and counting waterbird species (targets) in the Okavango Delta. We tested transferability to the Australian breeding colony. Our detection accuracy (targets), between the training and test data, was 91% for the Okavango Delta colony and 98% for the Lowbidgee floodplain colony. These estimates were within 1–5%, whether using freeware or payware for the different colonies. Our semi-automated method was 26% quicker, including development, and 500% quicker without development, than manual counting. Drone data of waterbird colonies can be collected quickly, allowing later counting with minimal disturbance. Our semi-automated methods efficiently provided accurate estimates of nesting species of waterbirds, even with complex backgrounds. This could be used to track breeding waterbird populations around the world, indicators of river and wetland health, with general applicability for monitoring other taxa.