In this work, valuable biocompatible Ag/Fe-enhanced TiO2 nanoparticles are comparatively prepared by a conventional wet chemistry method (sol-gel) and a rapid, efficient, hybrid unconventional method (microwave-assisted hydrothermal synthesis). In order to establish their application as effective compounds in sunscreens, the obtained powders were first structurally and morphologically characterized, analyses from which their nanodimensional character, crystalline structure and thermal behavior were highlighted. The evaluation of sunscreen effectiveness is based on the determination of the sun protection factor (SPF). It was observed that silver enhancing increases the SPF significantly, especially when compared to the pristine samples. The obtained Ag/Fe-enhanced TiO2 powders were also evaluated from the point of view of their biocompatibility on amniotic fluid stem cells, and the results indicated an enhance of cell proliferation when exposed to the synthesized nanostructures.