ABSTRACT Objective: to evaluate the quality of life of primary care nurses in the climacteric. Method: A cross-sectional descriptive-analytic study, performed with 98 female nurses, aged 40-65 years, using the WHOQOL-Bref questionnaire. Results: the worst level of quality of life was observed for professionals aged 50-59 years, non-white, specialists, divorced or widowed, with children, a lower income, with another employment relationship, a weekly workload of more than 40 hours, who consumed alcoholic beverages weekly, with chronic disease, in continuous use of medications, sedentary, who did not menstruate and did not receive hormonal treatment, and who went through menopause between the ages of 43-47 years. Conclusion: Although the variables “physical activity” and “age” have a statistically significant association with quality of life, other variables seem to interfere in these professionals’ lives, indicating the need for a more critical and deep reflection on these relations.