ABSTRACT Glyphosate has been widely used to control greater beggarticks populations that are resistant to acetolactate synthase (ALS) inhibitors in South America. However, herbicide control failures has been observed over the last three growing seasons in grain production areas of Paraguay. In this research, we report the first case of multiple resistance to glyphosate (EPSPs) and imazethapyr (ALS) in greater beggarticks (Bidens subalternans) population from Paraguay. This conclusion was supported by dose-response experiments conducted in two consecutive years (2018 and 2019) with a putative resistant (R) and a susceptible (S) population. Alternative herbicides were also tested for post-emergence control of R population. For glyphosate, the resistant factor (RF) values were 8.8- (2018) and 15.7-fold (2019). For imazethapyr, the RF values were 59- and 58-fold, in 2018 and 2019, respectively. Treatments with 2,4-D, dicamba, 2,4-D + glyphosate, dicamba + glyphosate, lactofen, fomesafen, ammonium-glufosinate, atrazine, and bentazon provided more than 80% control of the R population. This is the first case of multiple resistance to glyphosate and imazethapyr in greater beggarticks (Bidens subalternans) in the world. The mechanisms underlying resistance in this biotype should be evaluated in future research.