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Optica, Optics Express, 7(28), p. 9464, 2020

DOI: 10.1364/oe.388808

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Improving axial resolution of Bessel beam light-sheet fluorescence microscopy by photobleaching imprinting

Journal article published in 2020 by Bo Xiong, Xiaofei Han, Jiamin Wu ORCID, Hao Xie ORCID, Qionghai Dai ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Light-sheet microscopy has been widely used in high-speed fluorescence imaging with low phototoxicity, while the trade-off between the field-of-view and optical sectioning capability limits its application in large-scale imaging. Although Bessel beam light-sheet microscopy greatly enhances the light-sheet length with the self-healing ability, it suffers from the strong side-lobe effect. To solve these problems, we introduce the photobleaching imprinting technique in Bessel beam light-sheet microscopy. By extracting the non-linear photobleaching-induced fluorescence decay, we get rid of the large concentric side lobe structures of the Bessel beam to achieve uniform isotropic resolution across a large field-of-view for large-scale fluorescence imaging. Both numerical simulations and experimental results on various samples are demonstrated to show our enhanced resolution and contrast over traditional Bessel-beam light-sheet microscopy.