In order to investigate the shear capacity of prefabricated beam, experiments about shear bearing were performed on one integral pouring contrast beam and two prefabrication and assembly beams. Inspecting the relationship between load and deflection under the load, the development regularity of strain between longitude reinforced and stirrup, the shear capacity of cracking load and ultimate bearing capacity, analyzing failure mode of oblique section and connection performance of old and new concrete in the groove, and compared with the mechanical properties of cast-in-place concrete beam. The experimental results indicate that: prefabrication and assembly beam and cast-in-place beams have similar shear bearing capacity and failure mode of oblique section, along with the load level continues to increase, the groove joint stiffness weakened, but has little effect on the overall deformation.