AbstractThe assembly of species that colonize animal organic matter, their relative abundance, and dynamics are affected by the environmental and biogeographical conditions to which these resources are exposed. Baited trap studies are essential for research on the diversity, seasonality, distribution and population dynamics of necrophagous flies. Decomposing baits provide the necessary stimulus for flies to aggregate on them. In this study, three types of bait of animal origin with different organic chemical composition were compared in terms of the diversity, richness, abundance, and species composition of saprophagous flies species that were attracted to them. Bone-meal (BM), cow liver (CL), and rotten chicken viscera (CV) were used as bait to collect flies. In total, 3,387 Calliphoridae, Sarcophagidae, and Muscidae adult flies were collected. The most abundant species were Lucilia ochricornis (Wiedemann 1830), Chrysomya albiceps (Wiedemann 1819) (Diptera: Calliphoridae), Peckia (Sarcodexia) lambens (Wiedemann 1830) (Diptera: Sarcophagidae), and Ophyra capensis (Wiedemann 1818) (Diptera: Muscidae). The type of bait had significant effects on both the total richness (F2,18 = 57.08; P < 0.0001) and the effective number of species (F2,18 = 12.81; P = 0.0003) per trap. The average richness was higher in traps baited with chicken viscera, followed by cow liver and finally by bone-meal. The composition of cow liver and bone meal species constitute subsets of the species collected with chicken viscera, thus using the three baits would not increase the number of species detected. These results indicate that chicken viscera is the most efficient bait for testing or assessing necrophagous fly diversity.