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Revista de Chimie, 5(68), p. 1089-1094, 2017

DOI: 10.37358/rc.17.5.5618

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Assessment of a Maslinic Acid Derivative and its Metabolite in Rat Blood by Liquid Chromatography Coupled with Mass Spectrometry

This paper was not found in any repository; the policy of its publisher is unknown or unclear.
This paper was not found in any repository; the policy of its publisher is unknown or unclear.

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Abstract

A new rapid high-throughput liquid chromatography coupled with tandem mass spectrometry method (LC-MS/MS) was developed for the blood assessment of a maslinic acid derivative (Benzyl (2a, 3b) 2,3-diacetoxy-olean-12-en-28-amide) and its metabolite. Separation was performed using a Zorbax SB-C18 column with a mobile phase composition of methanol and 0.2% formic acid in water (78:22, v/v) in isocratic flow, with a flow rate of 0.6 mL/min. Detection was performed by multiple reaction monitoring (MRM) mode, monitoring the transitions 338.3; 526.6; 544.6; 527.5; 545.5 m/z derived from 646.6 m/z for the maslinic acid derivative and 409; 338.3; 203; 205; 526.5 m/z derived from 562.6 m/z for its metabolite, using atmospheric pressure chemical positive ionisation source. Single-step protein precipitation with methanol was used for preparation of rat blood samples. The method was validated with respect to selectivity, linearity (r ] 0.9937), within-run and between-run precision (CV [ 12.0%) and accuracy (bias [ 7.9%) over the ranges of 20.5 - 656 ng/mL blood for maslinic acid derivative, and 20 - 640 ng/mL blood for maslinic acid derivative metabolite. The analytical method is simple, fast, selective and is suitable for bioanalysis of maslinic acid derivative and its metabolite from rat blood.