The paper is focused on designing a novel silk fibroin-magnetite biomaterial scaffold for wounds healing in terms of: synthesis, physico-chemical and morphological characterization and in vitro biological behaviour assessment. Magnetic scaffolds were prepared from silk fibroin solutions and magnetic nanoparticles with various concentrations by solvent casting technique. Specimens were investigated by FTIR-ATR spectroscopy and XRD measurements. Morphological investigation including internal structure was employed by AFM and TEM/HRTEM analyses. Biological assay was performed on human adipose derived stem cells isolated from subcutaneous adipose tissue. Results suggested that all the tested magnetic silk-magnetite scaffolds display a good biocompatibility in vitro on hASCs and could be promising candidates for further wound dressing testing.