The objective of this work was to assess the influence of gas sampling frequency on the cumulative emissions of nitrous oxide (N2O) from the soil. Gas emissions were assessed over a period of two years (2014-2016), in four systems: eucalyptus forestry, crops, pasture, and native forest. The cumulative emissions of N2O were calculated at sampling intervals of 7, 14, and 21 days. The sampling intervals did not influence the final results of cumulative N2O emissions from the soil in the assessed systems.