This paper analyzes the harmonic contents of a non-linear load connected to a two-phase microgrid system. Although having the same harmonic content as the single-phase power system when supplying a non-linear load under balanced conditions, the two-phase microgrid system presents the following advantages: 1) constant power through the power line at the balanced condition; 2) two voltages i.e., line-to-line and phase voltages, available by using a three wire system; 3) optimized voltage utilization compared to a three-phase system; and 4) a direct connection of both symmetrical two-phase and single-phase electrical machines. This paper presents an approach for analyzing the harmonics of a two-phase non-linear load in a balanced and unbalanced cases. The mathematical model for the symmetrical component of an unbalanced two-phase system has also been presented in this paper. Finally, a practical implementation of the two-phase system has been performed, where different types of loads are connected to the two-phase power line to test the voltage control performance.