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American Association for the Advancement of Science, Science Advances, 11(5), 2019

DOI: 10.1126/sciadv.aaw9807

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The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation

Journal article published in 2019 by Qian Zhang ORCID, Fengcai Wen, Siqi Zhang, Jiachuan Jin, Lulu Bi ORCID, Ying Lu ORCID, Ming Li ORCID, Xu-Guang Xi ORCID, Xingxu Huang, Bin Shen ORCID, Bo Sun ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

This work determines the interactions between spCas9/sgRNA and DNA that dictate spCas9 binding and dissociation.