Abstract The analysis of spatial of national land development pattern is of great significance for optimizing the layout of spatial of national land development and realizing regional sustainable development. The comprehensive evaluation on spatial of national land pattern of Lanzhou-Xining urban agglomeration can deepen the scientific cognition of rational spatial of national land development of regional urban agglomeration and provides a theoretical basis for space governance. Based on the actual situation of Lanzhou-Xining urban agglomeration, this paper analyzed the carrying capacity of resource and environment in counties and districts, clarified the spatial difference of carrying capacity of resources and environment, and proposed the characteristics of land development pattern of Lanzhou-Xining urban agglomeration. As shown by the results, the spatial pattern of the natural carrying capacity index is distinctly different, showing a decreasing trend from the study area center to the periphery and also from the east to the west; High carrying capacity potential areas are mainly concentrated in Lanzhou, Xining and their surrounding counties; the spatial difference in the comprehensive index of resource and environment carrying capacity is large, and the contribution of natural carrying capacity to the resource and environment carrying capacity is higher, the spatial distribution of high value area is more discrete, and the spatial distribution of low value area has obvious agglomeration characteristics.