Published in

American Society for Microbiology, Journal of Clinical Microbiology, 7(51), p. 2040-2046, 2013

DOI: 10.1128/jcm.00304-13

Links

Tools

Export citation

Search in Google Scholar

PCR-Electrospray Ionization Mass Spectrometry for Direct Detection of Pathogens and Antimicrobial Resistance from Heart Valves in Patients with Infective Endocarditis

Journal article published in 2013 by Cassandra L. Brinkman, Paschalis Vergidis, James R. Uhl, Bobbi S. Pritt ORCID, Franklin R. Cockerill, James M. Steckelberg, Larry M. Baddour, Joseph J. Maleszewski, William D. Edwards, Rangarajan Sampath, Robin Patel
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

Full text: Download

Green circle
Preprint: archiving allowed
Upload
Green circle
Postprint: archiving allowed
Upload
Orange circle
Published version: archiving restricted
Upload
Policy details
Data provided by SHERPA/RoMEO

Abstract

ABSTRACT Microbiological diagnosis is pivotal to the appropriate management and treatment of infective endocarditis. We evaluated PCR-electrospray ionization mass spectrometry (PCR/ESI-MS) for bacterial and candidal detection using 83 formalin-fixed paraffin-embedded heart valves from subjects with endocarditis who had positive valve and/or blood cultures, 63 of whom had positive valvular Gram stains. PCR/ESI-MS yielded 55% positivity with concordant microbiology at the genus/species or organism group level (e.g., viridans group streptococci), 11% positivity with discordant microbiology, and 34% with no detection. PCR/ESI-MS detected all antimicrobial resistance encoded by mecA or vanA / B and identified a case of Tropheryma whipplei endocarditis not previously recognized.