Montrichardia linifera is commonly known in Brazil as “aninga”. It was collected two times (Nov/2008 and Aug/2009), and their leaves and stems were extracted with hexane or dichloromethane and methanol. Its biological and chemical potential, phytochemical profile and polyphenol content of their extracts were evaluated. There were found differences on the chemical composition: from the first collection, there were the apolar extracts the richest in metabolite composition and from the second collection the polar ones were the richest. Extracts from Aug/2009 showed, in general, antioxidant and cytotoxic activities higher than extracts from Nov/2008. Stems and leaves extracts from Aug/2009 collection showed higher polyphenols content. For the insecticide assay against Sitophilus zeamais, the hexane extract of leaves (Aug/2009) presented an increase in the insecticidal activity in a concentration dependent way. When evaluating the antibacterial potential, only methanolic extract of stems and leaves (Aug/2009) presented a small inhibition halo (9 and 7 mm, respectively) against Aeromonas hydrophila. The study shows that collection period is an important factor to be considered when the aims are phytochemical studies and biological activity of M. linifera.