Purpose: To examine the variations in the velocity of contraction (V_c) assessed using tensiomyography, vertical jumping ability, and sprinting speed induced by 4 different exercise protocols (ie, strength, sprint, plyometric, and technical training sessions) in 14 male national-team rugby players (age 21.8 [2.6] y, weight 83.6 [8.5] kg, and height 177.4 [6.7] cm). Methods: Physical tests were conducted immediately before and after 4 distinct workouts in the following order: tensiomyography in the rectus femoris and biceps femoris muscles, squat and countermovement jumps, and 30-m sprint velocity. To analyze the differences in the assessed variables before and after each training session, the differences based on magnitudes were calculated. Results: After strength and plyometric workouts, the players presented possible to almost certain impairments in sprint and jump performance and in the V_c of the rectus femoris (effect sizes 0.26–0.64). After the sprint-training session, possible to very likely decreases were observed in the squat jump, 30-m sprint, and V_c of the biceps femoris (effect sizes 0.21–0.44). By contrast, after the technical training, athletes demonstrated a possible increase in the squat jump and V_c in both muscles examined (effect sizes 0.13–0.20). Conclusions: The main finding of this research is that, for the vast majority of results, the direction of changes observed in V_c were the same as those observed in performance assessments. This suggests that V_c might be used as a sensitive marker of acute variations in speed and power performance of elite team-sport athletes.