Total Suspended Solids (TSS) and chlorophyll-a concentration are two critical parameters to monitor water quality. Since directly collecting samples for laboratory analysis can be expensive, this paper presents a methodology to estimate this information through remote sensing and Machine Learning (ML) techniques. TSS and chlorophyll-a are optically active components, therefore enabling measurement by remote sensing. Two study cases in distinct water bodies are performed, and those cases use different spatial resolution data from Sentinel-2 spectral images and unmanned aerial vehicles together with laboratory analysis data. In consonance with the methodology, supervised ML algorithms are trained to predict the concentration of TSS and chlorophyll-a. The predictions are evaluated separately in both study areas, where both TSS and chlorophyll-a models achieved R-squared values above 0.8.