This special issue focuses on highlighting the progress of last decade regarding the new nanostructured materials based on noble metals, especially gold and silver. Innovative preparations, functionalizations, and characterizations of these nanomaterials are investigated. Moreover, biotechnological applications, and advanced uses of these compounds for environmental sensing are reported. In particular gold and silver nanomaterials are widely studied due to their high stability, amazing chemical–physical features and, for silver, marked antibacterial properties. It is also hoped that the current special issue will encourage multidisciplinary research on noble metal nanomaterials, expanding the range of potential biological applications. This must be associated with improvements in synthetic methods and with economic feasibility studies of the proposed processes, also exploring the ecotoxicological aspects.