QTLs associated with ABA content in triticale anthers were analysed using the population of 72 DH lines derived from F1 cross between inbred line ‘Saka 3006’ and cv. ‘Modus’. Using Windows QTL Cartographer and MapQTL 5.0 softwares, composite interval mapping (CIM) and association analysis (Kruskal–Wallis test) for ABA content before and after low temperature treatment inducing androgenic development were conducted. The concentration of ABA in freshly cut tillers varied from 0.8 to 2.2 nmol g-1 DW. Intensive ABA accumulation (up to 5.3 nmol g-1 DW) was observed in response to low temperature stress. No correlation between ABA concentration and androgenesis induction efficiency was detected, whereas negative correlation (up to r = -0.33) was found between concentration of ABA in anthers collected from cold treated tillers and regeneration ability. CIM analysis indicated one QTL localized on chromosome 5A (LOD = 2.5; R2 = 9.3 %) associated with ABA concentration in unstressed plants and four QTLs on chromosomes 2A, 1B and 5R (LOD 2.6–5.7; R2 = 9.5–24 %) regulating ABA accumulation in response to low temperature stress. The Kruskal–Wallis test revealed significant (p<0.01) association with markers localized on chromosome 4A, 5A and 2RS.3R for unstressed plants and on chromosomes 3A, 3B, 5B and 2RS.3R for low temperature treated plants.