AbstractThere is a need for new targets to specifically localize inflammatory foci, usable in a wide range of organs. Here, we hypothesized that the cleaved molecular form of CD31 is a suitable target for molecular imaging of inflammation. We evaluated a bioconjugate of D-P8RI, a synthetic peptide that binds all cells with cleaved CD31, in an experimental rat model of sterile acute inflammation. Male Wistar rats were injected with turpentine oil into the gastrocnemius muscle two days before ^99mTc-HYNIC-D-P8RI (or its analogue with L-Proline) SPECT/CT or [¹⁸F]FDG PET/MRI. Biodistribution, stability study, histology, imaging and autoradiography of ^99mTc-HYNIC-D-P8RI were further performed. Biodistribution studies revealed rapid elimination of ^99mTc-HYNIC-D-P8RI through renal excretion with almost no uptake from most organs and excellent in vitro and in vivo stability were observed. SPECT/CT imaging showed a significant higher ^99mTc-HYNIC-D-P8RI uptake compared with its analogue with L-Proline (negative control) and no significant difference compared with [¹⁸F]FDG (positive control). Moreover, autoradiography and histology revealed a co-localization between ^99mTc-HYNIC-D-P8RI uptake and inflammatory cell infiltration. ^99mTc-HYNIC-D-P8RI constitutes a new tool for the detection and localization of inflammatory sites. Our work suggests that targeting cleaved CD31 is an attractive strategy for the specific in vivo imaging of inflammatory processes.