The aim of this study was to determine relationships between repeated 1 × 1 small-sided games (SSGs) (variable duration, constant work-to-rest ratio) and the concentration of steroid hormones and characteristic fatigue markers in youth soccer players. Eighteen young male soccer players were assigned at random to two experimental groups: E1—undertaking a six 30 s one-on-one SSGs with a 2 min rest period; and E2—playing six 45 s SSGs with a 3 min rest interval. Capillary blood was collected from the players at rest, after the last game, and 15 and 30 min after the exercise protocol. The variables assessed included serum cortisol (C), free testosterone (FT) and total testosterone (TT). An effect was observed between the measurement times (TT (F = 15.26, p ≤ 0.0001), FT (F = 6.86, p = 0.0006)). In terms of cortisol (C) levels, no interactions or effect between the studied groups were revealed, but an interaction was found (F = 4.01, p = 0.0126) and the effect appeared between the measurement times (F = 11.16, p ≤ 0.0001). The study results show that in all likelihood, longer rest intervals in repeated 30 s 1 × 1 SSGs can reduce catabolic reactions and hence the risk of overtraining in youth soccer players.