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eLife Sciences Publications, eLife, (4), 2015

DOI: 10.7554/elife.05808

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CaRuby-Nano: a novel high affinity calcium probe for dual color imaging

Journal article published in 2015 by Mayeul Collot, Christian D. Wilms, Asma Bentkhayet, Paikan Marcaggi, Kiri Couchman, Serge Charpak, Stéphane Dieudonné, Michael Häusser, Anne Feltz, Jean-Maurice Mallet ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments.