National Academy of Sciences, Proceedings of the National Academy of Sciences, 13(116), p. 6351-6360, 2019
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Significance Leishmania alternates between an insect vector and human host; in these different environments, the parasite adopts different forms. There are important commonalities between these different forms, particularly the flagellar pocket (FP) and associated flagellum attachment zone (FAZ). We show that the FAZ is important in different forms of Leishmania for FP shape and function, which are altered in mutants lacking a FAZ protein, FAZ5. FAZ5 deletion did not affect parasite proliferation and differentiation in culture; however, it dramatically reduced parasite proliferation in the sand fly and mouse. These results demonstrate the importance of the FAZ for FP function and architecture, and show that deletion of one FAZ protein can have a dramatic effect on Leishmania development and pathogenicity.