Future Medicine, Epigenomics, 3(11), p. 337-347, 2019
Elsevier, European Urology Supplements, 11(18), p. e3562, 2019
DOI: 10.1016/s1569-9056(19)34698-6
Full text: Unavailable
Aim: To analyze the potential of 14 cancer-associated genes, including six miRNAs, for bladder cancer (BC) diagnosis in urine. Patients & methods: DNA methylation levels of 14 genes were analyzed in urine of 72 BC patients and 75 healthy controls using quantitative methylation-specific PCR. Multivariate logistic regression analysis was used to determine an optimal marker panel. Results: Ten genes were significantly hypermethylated in BC patients. The GHSR/MAL combination showed the best diagnostic performance, reaching a sensitivity of 92% (95% CI: 86–99) and a specificity of 85% (95% CI: 76–94). Conclusion: We identified a novel two-gene panel with a high diagnostic accuracy for BC that can be applied in a noninvasive, urine-based test.