New data on the distribution, biology and ecology of the longhorn beetles occurring in southern and eastern regions of Kazakhstan are presented together with a list of 78 species that were collected during two entomological expeditions conducted in May and June 2017. New localities of some rare taxa endemic to this region of Asia, such asPsilotarsusbrachypterusbrachypterus(Gebler, 1830),Stenocorusminutus(Gebler, 1841) andDorcadioncrassipescrassipesBallion, 1878 are given.ExocentrusstierliniGanglbauer, 1883 is recorded from Kazakhstan for the first time. Moreover, the occurrence of three species:AmarysiusduplicatusTsherepanov, 1980,RhopaloscelisunifasciatusBlessig, 1873 andSaperdaalbertiPlavilstshikov, 1916, which were recently found in the country, is also confirmed. Furthermore, high-quality photographs of several unique taxa, i.e.Psilotarsusbrachypteruspubiventris(Semenov, 1900),Xylotrechusadspersus(Gebler, 1830),X.alakolensisKarpiński & Szczepański, 2018,Anoplistesgalusoi(Kostin, 1974),A.jacobsoniBaeckmann, 1904 andObereakostiniDanilevsky, 1988 along with images of their habitats and feeding galleries are also presented. New localities of species considered serious pests or invasive, such asTuraniumscabrum(Kraatz, 1882) andTrichoferuscampestris(Faldermann, 1835), respectively, are also given. A new synonymy is proposed:CerambyxscalarisLinnaeus, 1758 =CerambyxhieroglyphicusPallas, 1773,syn.n.