The use of biological techniques such as plant growth-promoting bacteria (PGPB) can represent a sustainable alternative for cereal growth in tropical areas. Research showing the potential for management practices which optimize PGPB inoculation is of utmost importance. This research was developed to investigate the potential use of Azospirillum brasilense in wheat cropping systems, as well as to assess the potential synergistic interactions between the beneficial use of silicon (Si), principally under abiotic and biotic conditions, and A. brasilense forms of application and how they impact crop development and wheat yield. The study was set up in a Rhodic Hapludox under a no-till system. The experimental design was a completely randomized block design with four replicates arranged in a factorial scheme with four inoculation forms (control, seed, groove, and leaf) and two soil acidity corrective sources (Ca and Mg silicate as Si source and dolomitic limestone). Seed inoculation was more effective in promoting wheat growth and development, with higher yield, showing an increase of 26.7% in wheat grain yield. Calcium and magnesium silicate application associated with foliar inoculation and without A. brasilense inoculation can increase wheat grain yield.