We address the life cycle of semantic web based knowledge management from ontology modelling to instance generation and re- use. We illustrate through a semantic web based knowledge manage- ment approach the potential of applying semantic web technologies in GEODISE, an e-Science pilot project in the domain of Engineering De- sign Search and Optimization (EDSO). In particular, we show how on- tologies and semantically enriched instances are acquired through knowledge acquisition and resource annotation. This is illustrated not only in Protégé with an OWL plug-in, but also in a light weight func- tion annotator customized for resource providers to semantically de- scribe their own resources to be published. In terms of reuse, advice mechanisms, in particular a knowledge advisor based on semantic matching, are designed to consume the semantic information and facili- tate service discovery, assembly and configuration in a real problem solving environment. An implementation has demonstrated the integra- tion of the advisor in a text mode domain script editor and a GUI mode workflow composition environment. Our research work shows the po- tential of using semantic web technology to manage and reuse knowl- edge in e-Science.